Hancock Tumor Glycomics Laboratory
Principal Investigator: William Hancock, Ph.D., Sc.D.
Institution: Northeastern University, Boston, MA
The Hancock Tumor Glycomics Laboratory applies an integrated glycoproteomics approach to discover glycan biomarkers for early detection of breast cancer.
This multi-disciplinary team of experts in glycomics, proteomics, informatics, and cancer markers focuses on the analysis and identification of glycoproteins in plasma and tissue and is developing analytical approaches to identify and characterize changes in the glycan associated with these glycoproteins. Candidate glycoprotein markers will be prioritized based on their elevated levels in both breast tumor tissue and plasma and the occurrence of altered glycosylation. To facilitate subsequent clinical studies, they plan to apply a high-throughput and sensitive glycan assay platform to pre-validate markers in a large sample set.
Another study involves determining the glycan structures of the secreted form of EGFR from A431 cell lines. This research may provide an understanding of glycosylation differences between secreted and plasma membrane forms of cancer-associated signaling molecules, which can be used to develop cancer biomarkers. This group has established collaborations with laboratories at the Fred Hutchinson Cancer Research Center and Indiana University .
Publications:
- Lu Q, Padler-Karavani V, Yu H, Chen X, Wu SL, Varki A, Hancock WS. LC-MS Analysis of Polyclonal Human Anti-Neu5Gc Xeno-Autoantibodies Immunoglobulin G Subclass and Partial Sequence Using Multistep Intravenous Immunoglobulin Affinity Purification and Multienzymatic Digestion. Anal Chem. 2012; (in press).
- Paik YK, Jeong SK, Omenn GS, Uhlen M, Hanash S, Cho SY, et al. A Chromosome-Centric Human Proteome Project to Characterize the Sets of Proteins Encoded in the Genome Nature Biotech., 2012; (in press).
- Rafalko A, Dai S, Hancock WS, Karger BL, Hincapie M. Development of a
Chip/Chip/SRM Platform Using Digital Chip Isoelectric Focusing and LC-Chip Mass
Spectrometry for Enrichment and Quantitation of Low Abundance Protein Biomarkers
in Human Plasma. J Proteome Res. 2012; 11:808-17.
- Tep S, Hincapie M, Hancock WS. A general approach for the purification and
quantitative glycomic analysis of human plasma. Anal Bioanal Chem. 2012; 402:2687-700.
- Zeng Z, Hincapie M, Pitteri SJ, Hanash SM, Schalkwijk J, Hogan JM, Wang H, Hancock W. A Proteomics Platform Combining Depletion, Multi-lectin Affinity Chromatography (M-LAC) and Isoelectric Focusing to Study the Breast Cancer Proteome. Anal Chem. 2011; 83:4845-54.
- Wang D, Hincapie M, Rejtar T, Karger BL. Ultrasensitive characterization of site-specific glycosylation of affinity purified haptoglobin from lung cancer patient plasma using 10 µm i.d. porous layer open tubular (PLOT) LC-LTQ-CID/ETD-MS. Anal Chem. 2011; 83(6):2029-37.
- Tousi F, Hancock WS, Hincapie, M. Technologies and strategies for glycoproteomics and glycomics and their application to clinical biomarker research. Anal. Methods. 2011; 3(1):20–32.
- Zeng Z, Hincapie M, Haab BB, Hanash S, Pitteri SJ, Kluck S, Hogan JM, Kennedy J, Hancock WS. The development of an integrated platform to identify breast cancer glycoproteome changes in human serum. J Chromatogr A. 2010 May 7; 1217(19):3307-15.
- Kullolli M, Hancock WS, Hincapie M. An automated platform for fractionation of human plasma glycoproteome in clinical proteomics. Anal Chem. 2010; 82(1):115-20.
- Taylor AD, Hancock WS, Hincapie M, Taniguchi N, Hanash SM. Towards an integrated proteomic and glycomic approach to finding cancer biomarkers. Genome Med. 2009; Jun 4, 1(6):57.
- Lu Q, Zheng X, McIntosh T, Davis H, Nemeth JF, Pendley C, Wu SL, Hancock WS. Development of different analysis platforms with LC-MS for pharmacokinetic studies of protein drugs. Anal Chem. 2009;Nov 1;81(21):8715-23.
- Ralin DW, Dultz SC, Silver JE, Kullolli M, Hancock WS, Hincapie M. Kinetic Analysis of glycoprotein-lectin interactions by label free internal reflection. Clin Proteomics. 2008;4,37-46.
- Kullolli M, Hancock WS, Hincapie M. Preparation of a high-performance multi-lectin affinity chromatography (HP-M-LAC) adsorbent for the analysis of human plasma glycoproteins. J Sep Sci. 2008; 14, 2733-9.
In the Media:
"Sweetening The Search For Breast Cancer Biomarkers", Chemical & Engineering News, May 2, 2011
"Major grants help researchers take on serious diseases and urban-health crises; William Hancock wants to catch breast cancer sooner," Northeastern University Alumni Magazine, Winter 2007/2008, vol. 33, no. 2.
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