Publications
Glycan Profile Analysis
2011
Zhuang Z, Mitra I, Hussein A, Novotny MV, Mechref Y, Jacobson S. Microchip Electrophoresis of N-Glycans on Serpentine Separation Channels with Asymmetrically Tapered Turns. Electrophoresis 2011; 32(2):246-53.
2010
Mann B, Madera M, Klouckova I, Dobrolecki LE, Hickey RJ, Hammoud ZT, Novotny MV. A Quantitative Investigation of Fucosylated Serum Glycoproteins with Application to Esophageal Adenocarcinoma. Electrophoresis 2010; 31, 1833-1841.
Alley WR Jr, Madera M, Mechref Y, Novotny MV. Chip-based reversed-phase liquid chromatography-mass spectrometry of permethylated N-linked glycans: a potential methodology for cancer-biomarker discovery. Anal Chem. 2010; 82(12):5095-106.
Alley WR, Novotny MV. Glycomic analysis of sialic acid linkages in glycans derived from blood serum glycoproteins. J Proteome Res. 2010; 9(6): 3062-72
Mann B, Madera M, Klouckova I, Mechref Y, Dobrolecki LE, Hickey RJ, Hammoud ZT, Novotny MV. A quantitative investigation of fucosylated serum glycoproteins with application to esophageal adenocarcinoma. Electrophoresis. 2010; 31(11):1833-41.
2009
Goetz JA, Mechref Y, Kang P, Jeng MH, Novotny, MV. Glycomic profiling of invasive and non-invasive breast cancer cells. Glycoconj J. 2009; 26(2):117-31.
Goldman R, Ressom HW, Varghese RS, Goldman L, Bascug G, Loffredo CA, Abdel-Hamid M, Gouda I, Ezzat S, Kyselova Z, Mechref Y, Novotny M.V. Detection of hepatocellular carcinoma using glycomic analysis. Clinical Cancer Res. 2009; 15(5):1808-13.
Mechref Y, Hussein A, Bekesova S, Pungpapong V, Zhang M, Dobrolecki LE, Hickey RJ, Hammoud ZT, Novotny MV. Quantitative serum glycomics of esophageal adenocarcinoma and other esophageal disease onsets. J Proteome Res. 2009; 8(6):2656-66.
2008
Isailovic D, Kurulugama RT, Plasencia MD, Stokes ST, Kyselova Z, Goldman R, Mechref Y, Novotny MV, Clemmer DE. Profiling of human serum glycans associated with liver cancer and cirrhosis by IMS-MS. J Proteome Res. 2008; 7:1109-1117.
Kyselova Z, Mechref Y, Kang P, Goetz JA, Dobrolecki LE, Sledge GW, Schnaper L, Hickey RJ, Malkas LH, Novotny, MV. Breast cancer diagnosis and prognosis through quantitative measurements of serum glycan profiles. Clin Chem. 2008; 54:1166-1175.
Ressom HW, Varghese RS, Goldman L, An Y, Loffredo CA, Abdel-Hamid M, Kyselova Z, Mechref Y, Novotny M, Drake SK, Goldman R. Analysis of MALDI-TOF mass spectrometry data for discovery of peptide and glycan biomarkers of hepatocellular carcinoma. J Proteome Res. 2008; 7:603-610.
2007
Kyselova Z, Mechref Y, Al Bataineh M, Dobrolecki L E, Hickey RJ, Sweeney CJ, Novotny MV. Alterations in the serum glycome due to metastatic prostate cancer. J Proteome Res. 2007; 6:1822-1832.
Zhuang Z, Starkey JA, Mechref Y, Novotny MV, Jacobson SC. Electrophoretic analysis of n-glycans on microfluidic devices. Anal Chem. 2007; 79:7170-7175.
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Glycoproteomics
2011
Zeng Z, Hincapie M, Pitteri SJ, Hanash SM, Schalkwijk J, Hogan JM, Wang H, Hancock W. A Proteomics Platform Combining Depletion, Multi-lectin Affinity Chromatography (M-LAC) and Isoelectric Focusing to Study the Breast Cancer Proteome. Anal Chem. 2011; 83:4845-54.
Wang D, Hincapie M, Rejtar T, Karger BL. Ultrasensitive characterization of site-specific glycosylation of affinity purified haptoglobin from lung cancer patient plasma using 10 µm i.d. porous layer open tubular (PLOT) LC-LTQ-CID/ETD-MS. Anal Chem. 2011; 83(6):2029-37.
2010
Zeng Z, Hincapie M, Haab BB, Hanash S, Pitteri SJ, Kluck S, Hogan JM, Kennedy J, Hancock WS. The development of an integrated platform to identify breast cancer glycoproteome changes in human serum. J Chromatogr A. 2010 May 7; 1217(19):3307-15.
Abbott KL, Lim JM, Wells L, Benigno BB, McDonald JF, Pierce M. Identification of candidate biomarkers with cancer-specific glycosylation in the tissue and serum of endometrioid ovarian cancer patients by glycoproteomic analysis. Proteomics. 2010; 10(3): 470-81.
2008
Abbott KL, Aoki K, Lim JM, Porterfield M, Johnson R, O'Regan RM, Wells L, Tiemeyer M, Pierce M. Targeted glycoproteomic identification of biomarkers for human breast carcinoma. J Proteome Res. 2008; 7(4):1470-80.
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Glycan/Glycopeptide Array Technologies
2012
Bovin NV, Obukhova P, Shilova N, Rapoport E, Popova I, Navakouski M, Unverzagt C, Vuskovic M, Huflejt M. Repertoire of human natural anti-glycan immunoglobulins. Do we have auto-antibodies? Biochim Biophys Acta. 2012; (In Press).
Vuskovic MI, Fang H, Pass HI, Huflejt ME. Optimal combination of glycan-based serum diagnostic markers which maximize AUC. J Systemics Cybernetics Informatics. 2012; (In Press).
Zhou X, Zhang J, Wang D. Microwave-assisted method for fabrication of carbohydrate cluster microarrays on 3-dimensional hydrazide-dendrimer substrate. Methods Mol Biol. 2012; 808:401-11.
Jacob F, Goldstein DR, Bovin NV, Pochechueva T, Spengler M, Caduff R, Fink D, Vuskovic MI, Huflejt ME, Heinzelmann-Schwarz V. Serum anti-glycan antibody detection of non-mucinous ovarian cancers by using a printed glycan array. Int J Cancer. 2012; 130:138-46.
2011
Pochechueva T, Jacob F, Goldstein DR, Huflejt ME, Chinarev A, Caduff R, Fink D, Hacker N, Bovin NV, Heinzelmann-Schwarz V. Comparison of printed glycan array, suspension array and ELISA in the detection of human anti-glycan antibodies. Glycoconj J. 2011; 28:507-17.
Vuskovic MI, Xu H, Bovin NV, Pass HI, Huflejt ME. Processing and analysis of serum antibody binding signals from Printed Glycan Arrays for diagnostic and prognostic applications. Int J Bioinf Res App. 2011; 7: 402-426.
Palma AS, Liu Y, Childs RA, Herbert C, Wang D, Chai W, Feizi T. The human epithelial carcinoma antigen recognized by monoclonal antibody AE3 is expressed on a sulfoglycolipid in addition to neoplastic mucins. Biochem Biophys Res Commun. 2011; 408:548-52.
Blixt O, Bueti D, Burford B, Allen D, Julien S, Hollingsworth M, Gammerman A, Fentiman I, Taylor-Papadimitriou J, Burchell JM. Autoantibodies to aberrantly glycosylated MUC1 in early stage breast cancer are associated with a better prognosis. Breast Cancer Res. 2011; 13(2):R25. [Epub ahead of print]
2010
Kracun SK, Cló E, Clausen H, Levery SB, Jensen KJ, Blixt O. Random glycopeptide bead libraries for seromic biomarker discovery. J Proteome Res. 2010; 9(12):6705-14.
Blixt O, Cló E, Nudelman AS, Sørensen KK, Clausen T, Wandall HH, Livingston PO, Clausen H, Jensen KJ. A high-throughput O-glycopeptide discovery platform for seromic profiling. J Proteome Res. 2010; 9(10):5250-61. Erratum in: J Proteome Res. 2011 10(3):1436.
Dunlop DC, Bonomelli C, Mansab F, Vasiljevic S, Doores KJ, Wormald MR, Palma AS, Feizi T, Harvey DJ, Dwek RA, Crispin M, Scanlan CN. Polysaccharide mimicry of the epitope of the broadly neutralizing anti-HIV antibody, 2G12, induces enhanced antibody responses to self oligomannose glycans. Glycobiology. 2010; 20(7):812-23.
Taylor RE, Gregg CJ, Padler-Karavani V, Ghaderi D, Yu H, Huang S, Sorenson RU, Chen X, Inostroza J, Nizet V, Varki A. Novel mechanism for the generation of human xeno-autoantibodies against the nonhuman sialic acid N-glycolylneuraminic acid. J. Exp. Med. 2010; 207:1637-1646.
Wandall HH, Blixt O, Tarp MA, Pedersen JW, Bennett EP, Mandel U, Ragupathi G, Livingston PO, Hollingsworth MA, Taylor-Papadimitriou J, Burchell J, Clausen H. Cancer biomarkers defined by autoantibody signatures to aberrant O-glycopeptide epitopes. Cancer Res. 2010; 70(4):1306-13.
2009
Carroll GT, Wang D, Turro NJ, Koberstein JT. Photogeneration of carbohydrate microarrays. In: ed. Dill K, Liu R, Grodzinski P. Microarrays: new development towards recognition of bio-signals. Springer, 2009.
Huflejt ME, Vuskovic MI, Vasiliu D, Xu H, Obukhova P, Shilova N, Tuzikov et al. Anti-carbohydrate antibodies of normal sera: findings, surprises and challenges. Mol Immunol. 2009; 46: 3037-3049.
Huang W, Wang D, Yamada M, Wang L. Chemoenzymatic synthesis and lectin array characterization of a class of N-glycan clusters. J Am Chem Soc 2009; 131(49):17963-71.
Zhou X, Turchi C, Wang D. Carbohydrate cluster microarrays fabricated on 3-dimensional dendrimeric platforms for functional glycomics exploration. J Proteome Res. 2009; 8(11): 5031-40.
2008
Carroll GT, Wang D, Turro NJ, Koberstein JT. Photons to illuminate the universe of sugar diversity through bioarrays. Glycoconj J. 2008; 25(1):5-10. Epub 2007 Jul 4.
Shilova NV, Galanina OE, Rubina AY, Butvilovskaya VI, Huflejt ME, Chambers J, Roucoux A, Bovin NV. 2-Aminopyridine ? a label for bridging of oligo-saccharides HPLS profiling and glycoarray printing. Glycoconj J. 2008; 25: 11-14.
Wang D, Gedela S. Insights of new tools in glycomics research [editorial]. J Proteomics Bioinform. 2008; 1(8): 374-378.
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Glycan Affinity Approaches
2011
Pejchal R, Doores KJ, Walker LM, Khayat R, Huang PS, Wang SK, Stanfield RL, Julien JP, Ramos A, Crispin M, Depetris R, Katpally U, Marozsan A, Cupo A, Maloveste S, Liu Y, McBride R, Ito Y, Sanders RW, Ogohara C, Paulson JC, Feizi T, Scanlan CN, Wong CH, Moore JP, Olson WC, Ward AB, Poignard P, Schief WR, Burton DR, Wilson IA. A Potent and Broad Neutralizing Antibody Recognizes and Penetrates the HIV Glycan Shield. Science 2011; 334:1097-103.
2010
Kullolli M, Hancock WS, Hincapie M. An automated platform for fractionation of human plasma glycoproteome in clinical proteomics. Anal Chem. 2010; 82(1):115-20.
2009
Diaz SL, Padler-Karavani V, Ghaderi D, Hurtado-Ziola N, Yu H, Brinkman-Van der Linden ECM, Varki A, Varki N.M. Sensitive and specific detection of the non-human sialic acid n-glycolylneuraminic acid in human tissues and biotherapeutic products. PLoS ONE. 2009; 4(1):e4241. Epub 2009 Jan 21.
Yue T, Goldstein IJ, Hollingsworth MA, Kaul K, Brand RE, Haab BB. The prevalence and nature of glycan alterations on specific proteins in pancreatic cancer patients revealed using antibody-lectin sandwich arrays. Mol Cell Proteomics. 2009; 8(7):1697-707. Epub 2009 Apr 17.
2008
Kullolli M, Hancock WS, Hincapie M. Preparation of a high-performance multi-lectin affinity chromatography (HP-M-LAC) adsorbent for the analysis of human plasma glycoproteins. J Sep Sci. 2008; 14, 2733-9.
Ralin DW, Dultz SC, Silver JE, Kullolli M, Hancock WS, Hincapie M. Kinetic analysis of glycoprotein-lectin interactions by label free internal reflection. Clin Proteomics. 2008;4,37-46.
2007
Wang D, Wu A. Carbohydrate microarrays for lectin characterization and glyco-epitope identification. In: Nilsson, CL, ed. Lectins: Analytical Technologies. Elsevier; 2007.
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Glycans and Oncogenesis
2011
Human xeno-autoantibodies against a non-human sialic acid serve as novel serum biomarkers and immunotherapeutics in cancer. Cancer Res. 2011; 71:3352-3363
2010
Guo HB, Johnson H, Randolph M, Nagy T, Blalock R, Pierce M. Specific posttranslational modification regulates early events in mammary carcinoma formation. Proc Natl Acad Sci U. S. A. 2010; 107(49):21116-21
2008
Abbott KL, Nairn AV, Hall EM, Horton MB, McDonald JF, Moremen KW, Dinulescu DM, Pierce M. Focused glycomic analysis of the N-linked glycan biosynthetic pathway in ovarian cancer. Proteomics. 2008; 8(16): p. 3210-20.
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Glycan Binding Proteins in Cancer
2011
SIGLEC12, a Human-specific Segregating (Pseudo)gene, Encodes a Signaling Molecule Expressed in Prostate Carcinomas. Mitra N, Banda K, Altheide TK, Schaffer L, Johnson-Pais TL, Beuten J, Leach RJ, Angata T, Varki N, Varki A. J Biol Chem. 2011; 286:23003-11.
2009
Thomas E, Newsom-Davis TE, Wang D, Steinman L, Chen PFT, Wang L, Simon AK, Screaton, GR. Enhanced immune recognition of cryptic glycan markers in human tumors. Cancer Res. 2009;69 (5):2018-2025.
2008
Costa NR, Mendes N, Marcos NT, Reis CA, Caffrey T, Hollingsworth MA, Santos-Silva F. Relevance of MUC1 mucin variable number of tandem repeats polymorphism in H pylori adhesion to gastric epithelial cells. World J Gastroenterol. 2008;14(9):1411-4.
Hedlund M, Padler-Karavani V, Varki N, Varki A. Evidence for a human-specific mechanism for diet and antibody-mediated inflammation in carcinoma progression. Proc Natl Acad Sci. 2008;105:18936-18941.
Padler-Karavani V, Yu H, Cao H, Chokhawala H, Karp F, Varki N, Chen X, Varki A. Diversity in specificity, abundance and composition of anti-neu5Gc antibodies in normal humans: potential implications for disease. Glycobiology. 2008;18:818-830.
2007
Swanson BJ, McDermott KM, Singh PK, Eggers JP, Crocker PR, Hollingsworth MA. MUC1 is a counter-receptor for myelin-associated glycoprotein (siglec-4a) and their interaction contributes to adhesion in pancreatic cancer perineural invasion. Cancer Res. 2007;67(21):10222-9.
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Reviews
2011
Padler-Karavani V, Varki A. Potential impact of the non-human sialic acid N-Glycolylneuraminic acid on transplant rejection risk. Xenotransplantation (Invited Commentary), 2011; 18:1-5.
Wang D. (2011). Carbohydrate Antigen Microarrays. In: ed. Chevolot Y. Carbohydrate Microarrays, Microarray Methods & Protocols, Humana Press, 2011; pp. 241-249.
Carroll GT, Wang D. Photoimmobilization of Saccharides, In: ed. Chevolot Y. Carbohydrate Microarrays, Microarray Methods & Protocols, Humana Press, 2011; (in press).
Tousi F, Hancock WS, Hincapie, M. Technologies and strategies for glycoproteomics and glycomics and their application to clinical biomarker research. Anal Methods. 2011; 3(1):20–32.
2010
Chen X, Varki A. Advances in the biology and chemistry of sialic acids. ACS Chem Biol. 2010;5(2):163-76.
2009
Taylor AD, Hancock WS, Hincapie M, Taniguchi N, Hanash SM. Towards an integrated proteomic and glycomic approach to finding cancer biomarkers. Genome Med. 2009;1(6):57.
2008
Wang D. Carbohydrate arrays. In: Matson, RS, ed. Microarray Methods & Protocols. 2008;CRC Press.
Liu Y, Palma AS , Feizi T. Carbohydrate microarrays: key developments in glycobiology. Biol Chem 2009;390:647-656.
Zhou X, Carroll GT, Turchi C, Wang D. Carbohydrate microarrays as essential tools of postgenomic medicine, In: Garg HG, Cowman MK, Hales CA, ed. Carbohydrate chemistry and biology for physicians. Elsevier, 2008.
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