Wang Tumor Glycomics Laboratory
Principal Investigator: Denong Wang, Ph.D.
Institution: SRI International (formerly Stanford Research Institute), Menlo Park, CA
Dr. Wang's long-term research interest is in the immunogenic sugar moieties that play key roles in the host recognition of complex carbohydrates and immune responses to carbohydrate antigens.
Dr. Wang's Alliance project extends to tumor glycomics with a current focus in exploration of glycan markers of prostate cancers and anti-glycan autoantibody signatures in prostate cancer patients. This is in partnership with Co-Investigator Ten Feizi at Imperial College London, UK. The Feizi group pioneered carbohydrate ligand discovery; the unique neoglycolipid technology coupled with mass spectrometry established by them was the basis of an advanced microarray system encompassing sequence-defined carbohydrate chains of glycoproteins and glycolipids. Among outputs are assignments of ligands for numerous endogenous carbohydrate recognition systems and those in pathogen host interactions including the oncogenic simian virus 40, SV40 and the pandemic H1N1 2009 influenza A virus.
The research team in the Alliance project is further strengthened by Stanford investigators with established research track records in prostate cancer, notably the establishment of the first international standard for detection of serum prostate-specific antigen and methodology for prostatic primary cultures; and in the development of flow cytometry (Hi-D FACS) for highly sensitive detection of cell and serum markers.
Key resources of the laboratory include large serum and tissue banks linked to extensive histopathologic and clinical databases with long follow up. Collaborating components of the team are: Denong Wang, Ph.D., Principal Investigator, SRI International; Leonard A. Herzenberg, Ph.D. and Leonore A. Herzenberg, Ph.D., Co-investigators, Stanford University (Biomarker Discovery); Ten Feizi, M.D., FmedSci., Co-Investigator, Imperial College London (Carbohydrate Analytics); Donna Peehl, Ph.D., Co-Investigator, Urology, Stanford University (Clinical Specimens); and Philip W. Lavori, Ph.D. Co-Investigator, Health Research and Policy, Biostatistics, Stanford University (Statistical Support).
Prior to joining the Alliance, Wang and his collaborators made considerable efforts to establish carbohydrate-based microarrays to facilitate identification and characterization of carbohydrate moieties of biomedical importance. His group published their first platform of carbohydrate microarrays in 2002. Subsequently, they developed technologies for construction of the epitope-specific glycan arrays. These include a method for photo-generation of oligosaccharide arrays and the most recent development of novel platforms of carbohydrate cluster microarrays. Their research activities using these technologies have led to the discovery of autoimmunogenic sugar moieties of SARS-CoV, a highly potent immunological target of Bacillus anthracis exosporium, and a number of cryptic glycan markers in human tumors.
Publications:
- Zhou X, Zhang J, Wang D. Microwave-assisted method for fabrication of carbohydrate cluster microarrays on 3-dimensional hydrazide-dendrimer substrate. Methods Mol Biol. 2012; 808:401-11.
- Pejchal R, Doores KJ, Walker LM, Khayat R, Huang PS, Wang SK, Stanfield RL, Julien JP, Ramos A, Crispin M, Depetris R, Katpally U, Marozsan A, Cupo A, Maloveste S, Liu Y, McBride R, Ito Y, Sanders RW, Ogohara C, Paulson JC, Feizi T, Scanlan CN, Wong CH, Moore JP, Olson WC, Ward AB, Poignard P, Schief WR, Burton DR, Wilson IA. A Potent and Broad Neutralizing Antibody Recognizes and Penetrates the HIV Glycan Shield. Science 2011; 334:1097-103.
- Palma AS, Liu Y, Childs RA, Herbert C, Wang D, Chai W, Feizi T. The human epithelial carcinoma antigen recognized by monoclonal antibody AE3 is expressed on a sulfoglycolipid in addition to neoplastic mucins. Biochem Biophys Res Commun. 2011; 408:548-52.
- Wang D. Carbohydrate Antigen Microarrays. In: ed. Chevolot Y. Carbohydrate Microarrays, Microarray Methods & Protocols, Humana Press, 2011; pp. 241-249.
- Carroll GT, Wang D. Photoimmobilization of Saccharides, In: ed. Chevolot Y. Carbohydrate Microarrays, Microarray Methods & Protocols, Humana Press, 2011 (In press).
- Dunlop DC, Bonomelli C, Mansab F, Vasiljevic S, Doores KJ, Wormald MR, Palma AS, Feizi T, Harvey DJ, Dwek RA, Crispin M, Scanlan CN. Polysaccharide mimicry of the epitope of the broadly neutralizing anti-HIV antibody, 2G12, induces enhanced antibody responses to self oligomannose glycans. Glycobiology. 2010; 20(7):812-23.
- Carroll GT, Wang D, Turro NJ, Koberstein JT. Photogeneration of carbohydrate microarrays. In: ed. Dill K, Liu R, Grodzinski P. Microarrays: new development towards recognition of bio-signals. Springer, 2009.
- Thomas E, Newsom-Davis TE, Wang D, Steinman L, Chen PFT, Wang L, Simon AK, Screaton, GR. Enhanced immune recognition of cryptic glycan markers in human tumors. Cancer Res. 2009;69 (5):2018-2025.
- Zhou X, Turchi C, Wang D. Carbohydrate cluster microarrays fabricated on 3-dimensional dendrimeric platforms for functional glycomics exploration. J Proteome Res. 2009; 8(11): 5031-40.
- Huang W, Wang D, Yamada M, Wang L. Chemoenzymatic synthesis and lectin array characterization of a class of N-glycan clusters. J Am Chem Soc. 2009; 131(49):17963-71.
- Liu Y, Palma AS , Feizi T. Carbohydrate microarrays: key developments in glycobiology. Biol Chem. 2009; 390:647-656.
- Carroll GT, Wang D, Turro NJ, Koberstein JT. Photons to illuminate the universe of sugar diversity through bioarrays. Glycoconj J. 2008; 25(1):5-10. Epub 2007 Jul 4.
- Zhou X, Carroll GT, Turchi C, Wang D. Carbohydrate microarrays as essential tools of postgenomic medicine, In: Garg HG, Cowman MK, Hales CA, ed. Carbohydrate chemistry and biology for physicians. Elsevier, 2008.
- Lucas AH, Rittenhouse-Olson K, Kronenberg M, Apicella M A, Wang D, Schreiber JR, Taylor CE. Carbohydrate moieties as vaccine candidates: Meeting summary. Vaccine. Jun 9, 2008. [Epub ahead of print]
- Wang D. "Carbohydrate arrays." In ed. Robert S. Mats: Microarray Methods & Protocols. CRC Press, 2008.
- Wang, D. and Gedela, S. (2008) Insights of new tools in glycomics research. J Proteomics & Bioinformatics (Editorial) 1(8): 374-378 (One of the most-accessed articles of JPB in 2008).
- Wang D, Carroll GT, Turro NJ, Koberstein JT, Kovác P, Saksena R, Adamo R, Herzenberg LA, Herzenberg LA, Steinman L. Photogenerated Glycan Arrays Identify Immunogenic Sugar Moieties of Bacillus anthracis Exosporium. Proteomics. 2007 Jan;7(2):180-4.
- Wang D, Wu A. Carbohydrate microarrays for lectin characterization and glyco-epitope identification. In: Nilsson, CL, ed. Lectins: Analytical Technologies. Elsevier; 2007.
In the Media:
"Stanford Tumor Glycome Laboratory – A New Initiative of University's Postgenomics Research," Research News, Stanford University Online Feature, October 18, 2007.
Patents
Prostate cancer glycan markers and autoantibody signatures. Inventors: Wang, D., Herzenberg, L. A., Peehl, D., Herzenberg, L. A. (US 7,981,625) July 19, 2011.
Provisional application: Cryptic glycan markers and applications thereof. Inventors: Newsom-Davis, T.E., Wang, D., Steinman, L., and Screaton, G. R. Filed: January 12, 2009.
Provisional application: Glycan markers and autoantibody signatures in HIV-1 and HIV-1-associated malignancies. Inventors: Wang, D. and Herzenberg, L. A. (2010). Filed: January 19, 2010
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